RIA or ELISA
Namely: radioimmunodiagnosis (RIA) or enzyme-linked immunoassay (ELISA) to detect anti-HCVIn serum In 1989, Kuo et al established the anti-C-100 radioimmunoassay (RIA), and then Ortho company successfully developed the enzyme-linked immunoassay (ELISA) to detect anti-C-100. In both methods, the recombinant yeast expressed viral antigen (C-100-3, the protein encoded by NS4, containing 363 amino acids) was purified and coated with a small amount of plastic plate pores, and then the serum was added. The viral antigen was bound to the anti-C-100 in the serum, and finally the mouse anti-human lgG monoclonal antibody was added with isotope or enzyme labeling. The result of substrate color rendering judgment.