The antigen of human serum amyloid A (SAA) is SAA protein itself, and the recombinant protein of SAA protein can be prepared by purifying SAA protein or genetic engineering technology as the antigen. Antibodies to SAA can be prepared by immunizing animals (such as mice, rabbits, etc.). The method of preparing antibodies involves injecting the SAA protein into an animal to produce an immune response, and then collecting antibodies from inside or outside the animal. Depending on the type and application of the antibody, you can choose to prepare polyclonal antibodies or monoclonal antibodies. Polyclonal antibody can recognize multiple epitopes of SAA protein, but has certain batch difference and cross-reactivity. Monoclonal antibodies have higher specificity and stability, but the preparation cost is higher. Antigens and antibodies of SAA can be used for the detection and quantification of SAA, such as through ELISA and other technologies.